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Enhance Your Testosterone Levels With Isatori Isa Test GF

Isa Test GF is Isatori's popular anabolic supplement for increasing testosterone, inhibiting estrogen, increasing strength and muscle mass, and also enhancing libido and sexual drive. The supplement contains several key ingredients that work to achieve these results with little to no side effects, and some of these ingredients include ZMA, Testofen (Fenugreek), Tribulus, Tongkat Ali (Eurycoma Longifolia), Epimedium Sagittatum (horny goat weed), and Ajuga Turkestanica (Turkesterone). Many of these ingredients are commonly found in various other testosterone enhancing supplements.

In my many years of training and supplement use, I've used my fair share of testosterone enhancing supplements such as BSN Axis HT, MHP T-Bomb II, Biotest Tribex, ZMA's, and Tribulus supplements just to name a few. I would have to give Isa-Test GF a two thumbs up, and rank it up there as one of the better test enhancers that gives good results. There are some interesting ingredients in Isatest GF, and in our review, we'll discuss some of the in some detail. If you've used Isa-Test, please share your review of Isa-Test GF here.

Isatori Isa-Test GF Ingredients   

Below is a list of the Isa Test GF ingredients:

104 Capsules
Serving Size4Capsules
Servings Per Container26
Amount Per Serving % DV*

Vitamin B6 (Pyridoxine HCl) 7mg 538%
Vitamin C (As Ascorbic Acid) 25mg 28%
Zinc (As Zinc Aspartate) 20mg 182%
Magnesium (As Oxide & Aspartate) 300mg 71%

Isa-Test, A Proprietary Blend Containing: 1714mg *
TESTOFEN (Trigonella Foenum Graecum Extract Standardized For 50% Fenuside Saponins), Tribulus Terrestris Extract (60% Total Saponins / 20% Protodioscin), Eurycoma Longifolia 20:1 Extract, Epimedium Sagittatum & Brevicornum Extract (Standardized For 10% Icariin), P-450 Anti-Aromatase [(S)-2,3- Dihydro-5,7-Dihydroxy-2-(3-Hydroxy-4-
Methoxyphenyl)-4-H-1benzopy-Ran-4-One], Ajuga Turkestanica & Rhaponticum Carthamoides Root Extract (Standardized For 11,20 Dihydroxyecdysone, 20-Beta-Hydroxyecdysone And 25-Deoxy-20- Hydroxyecdysone), Grapeseed Extract (95% Oligomeric Proanthocyanidins) Isaflow Microcirculation/nutrient Delivery System [Specially Prepared Hesperidin Methyl Chalcone, Ruscus (20% Sterolic Heterosides) & Vitamin C Complex]
SOMATRIN 325mg *
Nettle Extract (95% Divanillyltetrahydrofuran), Mucuna Pruriens (Standardized For 15% L-Dopa), N-Acetyl-5-Methoxytryptamine (Melatonin)

ZMA is one of the first ingredients listed. I think most probably know what ZMA is by now - Zinc Aspartate, Magnesium Aspartate, and Vitamin B6. Next up is Testofen, or Trigonella Foenum Graecum, an ingredient in Isa-Test's proprietary blend. Testofen is also known as Fenugreek seed extract. Fenugreek is often used as both a herb and a spice, and is commonly used in making Curry. It's had a long history of medicinal use in Ayurvedic and Chinese medicines. It has been used to help digestion, treat metabolic dysfunctions, and used as a general tonic to improve health. There are also numerous studies done which have found Fenugreek to:

  • considerably enhance testosterone levels in rats, decreased sperm shape abnormality, and also improved sperm count [1]
  • human studies have found consuming fenugreek seed extract decreases dietary fat consumption in humans [2]
  • Fenugreek significantly increased satiety and reduced energy intake in obese human subjects [3]
  • helps reduce daily fat consumption in overweight in overweight subjects [4]
  • Fenugreek seeds can be beneficial for type 2 diabetes [5]
  • studies using mice have found Fenugreek causes an increase in the utilization of fatty acids as an energy sources, which led to improved endurance and a decrease in fat accumulation [6]
  • Fenugreek extract significantly increases post-exercise glycogen resynthesis in human subjects [7]
  • Fenugreek seed extract containing steroid saponins increased food consumption and the motivation to eat in rats, and reduced plasma cholesterol levels in rats [8]

There is also a study or two done by Gencore Pacific Inc., floating around that's done on Testofen using human subjects showing a 98.81% increase in free testosterone levels, and a skin-fold thickness reduction of 20.18% and 14.64% for Triceps and Thighs respectively, indicating a reduction in body fat. [9] The large increase in free testosterone levels coupled with potential fat reduction sounds swell, but I would seriously take these test results with a grain of salt, or maybe with a handful of salt. I don't believe this study was published in any peer reviewed publications, at least none that I could find.

Tribulus Terrestris is another ingredient in the proprietary blend of Isatori Isa Test GF. This is one ingredient that has been studied widely, and there are contradicting results regarding the positive benefits of supplementing Tribulus Terrestris. There are a large number of studies that provide contradicting results on the effectiveness of tribulus. Various studies on rats have demonstrated that tribulus increased testosterone and sexual activity [10, 11], while other studies found that tribulus had no effect on muscle strength, body composition, exercise performance in human subjects [12, 13]. Obviously, taking these studies at face value, the studies involving human subjects would obviously carry more weight compared to the studies involving rats and other animals.

From personal experience, and based on user reviews of various tribulus based supplements, I would have to say that tribulus works, but not for everyone. Enough users have submitted positive reviews to show that tribulus based supplements do work. I can say from my past experiences that each time I've taken a tribulus supplement, I've noticed a difference - these include increased acne breakouts, and increased libido etc... Please keep in mind here, that my take on tribulus is purely personal opinion, and only based on my own past and present experiences of using various tribulus based supplements. It by no means, is a verdict on the effectiveness of the herb.

Eurycoma Longifolia (Tongkat Ali) - Tongkat ali, latin name Eurycoma longifolia, is a root that grows in the rainforests of Southeast Asia. Similar to Tribulus, the herb works by affecting the lutenizing hormones in the body, which signal testosterone production. Various studies in rats have shown Tongkat Ali to increase sex drive and libido. [14] Studies have also found Tongkat Ali to enhance male fertility by increasing semen volume, sperm concentrations, the percentage of normal sperm morphology, and sperm motility. One study of 350 patients given 200mg of the extract daily for 9 months found the patients to have significant improvement in semen quality. [15]

Epimedium Sagittatum (horny goat weed) - you can read more about Horny Goat Weed here.

One very interesting ingredient of Isatori Isa-Test GF is Turkesterone, also known as Ajuga Turkestanica. It is a plant extract harvested from Rhaponticum cathamoides - a thistle like plant that grows in Southern Siberia, Kazakhstan, Bulgaria and Central Asia. It is the most powerful beta ecdysterones discovered so far. Ecdysterones are plant sterols with anabolic effects with no known side effects.

Some of the effects of Turkesterone include [16]:

  • Increased production of both fast- and slow-twitch muscle fibres
  • Increased body weight due to improved muscle-to-fat ratio
  • Stimulates protein synthesis
  • Enhanced ATP and PCr synthesis
  • Increased muscle glycogen concentrations
  • Enhanced removal of lactic acid
  • No side effects at all have been observed [16]

With Turkesterone, there is a limited number of research done on lab animals. However, these studies have discovered some interesting effects of Turkesterone. Studies have found phytoecdisteroids such as Turkesterone stimulates protein synthesis and increases the rate of protein formation. [17] Turkesterone is found to have very similar effects to the anabolic steroid known as Nerobol. One study reviewing the effects of Turkesterone and Nerobol found that protein synthesis was stimulated in mouse liver tissue. [18] Another Russian study found that turkesterone caused a marked effect on red blood cell regeneration, and this ability of turkesterone to cause erythropoiesis (process of producing red blood cells) makes it very similar to the anabolic drug Nerobol. [19]

The final component of IsaTest GF is 325mg of Somatrin, which contains Nettle Extract, Mucuna Pruriens, and Melatonin. The Isatori webpage for Isa Test makes reference to a study showing Melatonin enhances exercise induced GH secretion in adult male subjects. I should point out here that the study is done on Melatonin, and only Melatonin, and not Somatrin itself, which includes Melatonin and 2 other ingredients. This study involved 7 healthy male subjects, and it was found that a single dose of 5mg melatonin significantly increased exercise induced GH secretion. [20]

Isatori Isa Test GF Review & Side Effects

There aren't many Isa Test side effects to mention. Probably one of the most common side effect is slightly increased aggression - something many users have reported, and I can share my own anecdote on that as well. Isatest will not make you aggressive all the time, but there seems to be times when you feel more aggressive and display more aggression. You may also notice increased acne breakouts as well.

On the plus side, Isatest does lead to strength and mass gains. I was training with much higher focus and intensity in the gym, and had much better overall endurance as well. There are users who have reported improved recovery time, however, I can't say that I had any better recovery times. On another note, the wife was quite happy while I was taking Isa Test GF. The libido increase was very noticeable, and erection frequency also increased quite significantly. =)

>> Click here for Isatori Isa Test GF


1. Arch Physiol Biochem. 2010 May 28. [Epub ahead of print]
Potential protective effect on key steroidogenesis and metabolic enzymes and sperm abnormalities by fenugreek steroids in testis and epididymis of surviving diabetic rats.
Hamden K, Jaouadi B, Carreau S, Aouidet A, El-Fazaa S, Gharbi N, Elfeki A.

Laboratory of Animal Ecophysiology, Faculty of Science of Sfax, PO Box 95, Sfax 3052, Tunisia.

2. Eur J Clin Pharmacol. 2009 Dec;65(12):1175-8. Epub 2009 Oct 7.
A fenugreek seed extract selectively reduces spontaneous fat consumption in healthy volunteers.
Chevassus H, Molinier N, Costa F, Galtier F, Renard E, Petit P.

3. Phytother Res. 2009 Nov;23(11):1543-8.
Effect of fenugreek fiber on satiety, blood glucose and insulin response and energy intake in obese subjects.
Mathern JR, Raatz SK, Thomas W, Slavin JL.

4. Eur J Clin Pharmacol. 2010 May;66(5):449-55. Epub 2009 Dec 18.
A fenugreek seed extract selectively reduces spontaneous fat intake in overweight subjects.
Chevassus H, Gaillard JB, Farret A, Costa F, Gabillaud I, Mas E, Dupuy AM, Michel F, Cantié C, Renard E, Galtier F, Petit P.

5. Int J Vitam Nutr Res. 2009 Jan;79(1):34-9.
Effect of fenugreek seeds on blood glucose and lipid profiles in type 2 diabetic patients.
Kassaian N, Azadbakht L, Forghani B, Amini M.

6. J Nutr Sci Vitaminol (Tokyo). 2006 Aug;52(4):287-92.
Effects of fenugreek seeds (Trigonella foenum greaecum) extract on endurance capacity in mice.
Ikeuchi M, Yamaguchi K, Koyama T, Sono Y, Yazawa K.

7. Amino Acids. 2005 Feb;28(1):71-6. Epub 2004 Dec 2.
The addition of fenugreek extract (Trigonella foenum-graecum) to glucose feeding increases muscle glycogen resynthesis after exercise.
Ruby BC, Gaskill SE, Slivka D, Harger SG.

8. Steroids. 1995 Oct;60(10):674-80.
Steroid saponins from fenugreek seeds: extraction, purification, and pharmacological investigation on feeding behavior and plasma cholesterol.
Petit PR, Sauvaire YD, Hillaire-Buys DM, Leconte OM, Baissac YG, Ponsin GR, Ribes GR.

9. Testofen Human Clinical Trial
Gencor Pacific, Inc. 2006

10. The hormonal effects of Tribulus terrestris and its role in the management of male erectile dysfunction - an evaluation using primates, rabbit and rat.
Gauthaman K, Ganesan AP.

11. Aphrodisiac properties of Tribulus Terrestris extract (Protodioscin) in normal and castrated rats.
Gauthaman K, Adaikan PG, Prasad RN.

12. The effect of five weeks of Tribulus terrestris supplementation on muscle strength and body composition during preseason training in elite rugby league players.
Rogerson S, Riches CJ, Jennings C, Weatherby RP, Meir RA, Marshall-Gradisnik SM.

13. The effects of Tribulus terrestris on body composition and exercise performance in resistance-trained males.Antonio J, Uelmen J, Rodriguez R, Earnest C.

14. Arch Pharm Res. 2001 Oct;24(5):437-40 Effects of Eurycoma longifolia jack on laevator ani muscle in both uncastrated and testosterone-stimulated castrated intact male rats.

15. Asian J Androl. 2010 May;12(3):376-80. Epub 2010 Mar 29.
Eurycoma longifolia Jack in managing idiopathic male infertility.
Tambi MI, Imran MK.

16. Turkesterone - The Most Powerful Ecdysterone So Far Discovered, Oct 12, 2009 -

17. Nauchnye Doki Vyss Shkoly Biol Nauki. 1984;(11):16-20.
[Mechanism of the anabolic action of phytoecdisteroids in mammals]
Syrov VN.

18. Vopr Med Khim. 1978 Jul-Aug;24(4):456-60.
[Effect of turkesterone and nerobol on the activity of the protein synthesizing system of mouse liver]
Syrov VN, Kurmukov AG, Sakhibov AD

19. Eksp Klin Farmakol. 1997 May-Jun;60(3):41-4.
[The results of experimental study of phytoecdysteroids as erythropoiesis stimulators in laboratory animals]
Syrov VN, Nasyrova SS, Khushbaktova ZA.

20 Eur J Endocrinol. 1999 Jul;141(1):22-6.
Exercise-induced GH secretion is enhanced by the oral ingestion of melatonin in healthy adult male subjects.
Meeking DR, Wallace JD, Cuneo RC, Forsling M, Russell-Jones DL.


June 2010